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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cells for FeFAB assay were kind gifts from Dr Martin Löchelt, German Cancer Research Center, Germany.

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。

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